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Product
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VEGF164, Rat (P. pastoris-expressed)
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Cat#
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101-26-10A
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Sequence
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MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI
MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPC
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC
DKPRR
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Unit/Weight
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2 μg
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Unit Price
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$32.00
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Description
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Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor[1], is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties[2]. During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188[3] which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR[4] or to the non-tyrosine kinase neuropilin receptors NRP1/2[5].
Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rrVEGF-A164 has a molecular mass of 38 kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
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Molecular Weight
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38 kDa, observed by non-reducing SDS-PAGE.
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Purity
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> 95% as analyzed by reducing SDS-PAGE.
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Storage
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Lyophilized recombinant rat Vascular Endothelial Growth Factor A164(rrVEGF-A164) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrVEGF-A164 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
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