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Product
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TNF-α, Mouse (P. pastoris-expressed)
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Cat#
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101-16-13B
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Sequence
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SQNSSDKPVA HVVANHQVEE QLEWLSQRAN ALLANGMDLK
DNQLVVPADG LYLVYSQVLF KGQGCPDYVL LTHTVSRFAI
SYQEKVNLLS AVKSPCPKDT PEGAELKPWY EPIYLGGVFQ
LEKGDQLSAE VNLPKYLDFA ESGQVYFGVI AL
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Unit/Weight
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100 μg
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Unit Price
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$252.00
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Description
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Sharing 79% sequence identity with human Tumor necrosis factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4].
Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
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Molecular Weight
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17kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% as analyzed by reducing SDS-PAGE.
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Storage
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Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-αshould be stable up to 1 week at 4°C or up to 2 months at -20°C.
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