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Product
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PDGF-BB, Mouse
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Cat#
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101-23-06B
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Sequence
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MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV
WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR
KKPIFKKATV TLEDHLACKC ETIVTPRPVT
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Unit/Weight
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50 μg
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Unit Price
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$188.00
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Description
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Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.
Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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24.7 kDa, observed by non-reducing SDS-PAGE.
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Purity
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> 95% by SDS-PAGE analysis.
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Storage
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Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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