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Product
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UBE2B/HR6B, Human
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Cat#
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101-32-139B
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Sequence
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MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI
FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN
VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
PANSQAAQLY QENKREYEKR VSAIVEQSWN DS
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Unit/Weight
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50 μg
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Unit Price
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$62.00
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Description
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Ubiquitin璫onjugating Enzyme E2 B (UBE2B), known as Homolog of Rad6B (HR6B), is a member of the Ubiquitin璫onjugating (E2) enzyme family. It is ubiquitously expressed with a predicted molecular weight of 17 kDa. HR6B/UBE2B localizes to the nucleus and cytoplasm and has an E2 catalytic core domain that forms a thioester bond with Ubiquitin. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.
Recombinant Human UBE2B produced in E. coli cells is a single non-glycosylated polypeptide chain ontaining 152 amino acids. A fully biologically active molecule, rhUBE2B has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
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Molecular Weight
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17.3 kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% as analyzed by SDS-PAGE&HPLC.
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Storage
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Lyophilized recombinant Human UBE2B/HR6B remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human UBE2B/HR6B should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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