Product
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H3N2 (A/Aichi/2/1968), Hemagglutinin
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Cat#
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101-32-99A
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Sequence
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QDLPGNDNST ATLCLGHHAV PNGTLVKTIT DDQIEVTNAT
ELVQSSSTGK ICNNPHRILD GIDCTLIDAL LGDPHCDVFQ
NETWDLFVER SKAFSNCYPY DVPDYASLRS LVASSGTLEF ITEGFTWTGV
TQNGGSNACK RGPGSGFFSR LNWLTKSGST YPVLNVTMPN
NDNFDKLYIW GIHHPSTNQE QTSLYVQASG RVTVSTRRSQ
QTIIPNIGSR PWVRGLSSRI SIYWTIVKPG DVLVINSNGN LIAPRGYFKM
RTGKSSIMRS DAPIDTCISE CITPNGSIPN DKPFQNVNKI
TYGACPKYVK QNTLKLATGM RNVPEKQTRG LFGAIAGFIE
NGWEGMIDGW YGFRHQNSEG TGQAADLKST QAAIDQINGK LNRVIEKTNE
KFHQIEKEFS EVEGRIQDLE KYVEDTKIDL WSYNAELLVA
LENQHTIDLT DSEMNKLFEK TRRQLRENAE EMGNGCFKIY
HKCDNACIES IRNGTYDHDV YRDEALNNRF QIKGVELKSG YKDWHHHHHH
HH
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Unit/Weight
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10 μg
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Unit Price
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$32.00
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Description
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Influenza hemagglutinin (HA) is a glycoprotein found on the surface of the influenzavirus. It is responsible for binding the virus to cells with sialic acid on their membranes, such as cells in the upper respiratory tract or erythrocytes. It is also responsible for the fusion of the viral envelope with the endosome membrane after the pH has been reduced. The name "hemagglutinin" comes from the protein's ability to cause red blood cells (erythrocytes) to clump together in vitro. HA has two functions. First, it allows the recognition of target vertebrate cells, accomplished through binding to these cells' sialic acid-containing receptors. Second, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. H3N2 is a subtype of influenza virus A and the most common cause of influenza in humans.
Recombinant Influenza A H3N2 (A/Aichi/2/1968) Hemagglutinin with his-tag produced in Sf9 Cells is a single, glycosylated polypeptide chain containing 522 amino acids. A fully biologically active molecule, HA-H3N2 has a molecular mass of ~66 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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~66 kDa, observed by reducing SDS-PAGE.
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Purity
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>95% by SDS-PAGE and HPLC analyses.
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Storage
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Lyophilized recombinant Influenza A H3N2 (A/Aichi/2/1968) Hemagglutinin remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, HA-H3N2 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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