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Product
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UBE2C/UBCH10, His, Human
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Cat#
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101-32-35B
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Sequence
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MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR
GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR
YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV
RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE
P
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Unit/Weight
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50 μg
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Unit Price
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$668.00
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Description
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Human Ubquitin Conjugating Enzyme E2 C (UBE2C)/UBCH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.
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Molecular Weight
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Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 ?His tag at N-terminus.
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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