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Product
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Hirudin
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Cat#
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101-32-18A
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Sequence
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VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV
TGEGTPGPQS HNDGDFEEPE EYL
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Unit/Weight
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10 μg
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Unit Price
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$128.00
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Description
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Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.
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Molecular Weight
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Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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