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Product
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SPARC, Human
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Cat#
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101-32-16B
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Sequence
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MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE
VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE
NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE
KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC
KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT
EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF
PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF
FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI
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Unit/Weight
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1 mg
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Unit Price
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$1243.00
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Description
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SPARC, an acronym for ‘secreted protein, acidic and rich in cysteine', is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
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Molecular Weight
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Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 ?His tag).
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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