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Product
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NT-4, Human
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Cat#
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101-30-09B
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Sequence
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MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE
VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR
GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC
VCTLLSRTGR A
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Unit/Weight
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10 μg
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Unit Price
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$68.00
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Description
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Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.
Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.
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Purity
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> 95% by SDS-PAGE and HPLC analyses.
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Storage
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Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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