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Product
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IGF-II, Human
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Cat#
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101-22-09B
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Sequence
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MAYRPSETLC GGELVDTLQF VCGDRGFYFS RPASRVSRRS
RGIVEECCFR SCDLALLETY CATPAKSE
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Unit/Weight
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50 μg
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Unit Price
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$78.00
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Description
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Insulin-like Growth Factor II (IGF-II) is a single chain 7 kDa polypeptide, and shares a high degree of homology with insulin. During circulation in vivo, IGF-II is complexed to high affinity binding proteins, IGF Binding Proteins (IGFBP), which act as circulating reservoirs, transport IGF-II, and prolong the half life of IGF-II. The receptors of IGF-II (IGFRs) are transmembrane tyrosine receptors, and are heterotetrameric consisting of two α-subunits and two β-subunits. IGFRs execute their role via intracellullar signaling molecules, such as IRS, shc, and PI3K. The functions of IGF-II include promoting cell survival, growth, proliferation, differentiation and motility. In particular, IGF-II promotes proliferation, inhibits death, and stimulates transformation in breast cancer cells.
Recombinant human Insulin-like Growth Factor II (rhIGF-II) produced in E. coli is a single non-glycosylated polypeptide chain containing 68 amino acids. A fully biologically active molecule, rhIGF-II has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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7.6 kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% as analyzed by SDS-PAGE and HPLC.
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Storage
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Lyophilized recombinant human Insulin-like Growth Factor II (rhIGF-II) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIGF-II remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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