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Product
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G-CSF, Rat
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Cat#
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101-21-42A
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Sequence
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MIPLLTVSSL PPSLPLPRSF LLKSLEQVRK IQARNTELLE
QLCATYKLCH PEELVLFGHS LGIPKASLSS CSSQALQQTK
CLSQLHSGLF LYQGLLQALA GISSELAPTL DMLHLDVDNF
ATTIWQQMES LGVAPTVQPT QSTMPIFTSA FQRRAGGVLV
TSYLQSFLET AHHALHHLPR PAQKHFPESL FISI
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Unit/Weight
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10 μg
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Unit Price
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$78.00
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Description
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Granulocyte Colony-Stimulating Factor (G-CSF) is a hematopoietic cytokine belonging to the four-helix bundle cytokine superfamily. G-CSF is produced by monocytes, macrophages, fibroblasts, and endothelial cells. Its expression is highly regulated and induced by a variety of agents, including Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interferon γ (IFN-γ), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). G-CSF binds to the CSF-specific high affinity receptors expressed on neutrophilic granulocyte lineage. In vivo G-CSF regulates the production of neutrophilic granulocytes, a critical part of host defense systems, and helps the maturation of leukemic cell lines. G-CSF is widely employed clinically because of its fairly innocuous safety profile.
Recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) produced in E. coli is a single non-glycosylated polypeptide chain containing of 194 amino acids. A fully biologically active molecule, rrG-CSF has a molecular mass of 21.4 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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21.4 kDa, observed by non-reducing SDS-PAGE.
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Purity
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> 95% by SDS-PAGE analysis.
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Storage
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Lyophilized recombinant rat Granulocyte Colony-Stimulating Factor (rrG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrG-CSF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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