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Product
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M-CSF, Human
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Cat#
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101-21-13B
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Sequence
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MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD
QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ
LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN
VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
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Unit/Weight
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100 μg
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Unit Price
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$443.00
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Description
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Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis,[1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63.
Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at Pepmic.
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Molecular Weight
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28 kDa, observed by non-reducing SDS-PAGE.
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Purity
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> 95% as analyzed by non-reducing SDS-PAGE.
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Storage
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Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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