|
Product
|
FGF-8c, Mouse
|
|
Cat#
|
101-19-53A
|
|
Sequence
|
MQVRSAAQKR GPGAGNPADT LGQGHEDRPF GQRSRAGKNF
TNPAPNYPEE GSKEQRDSVL PKVTQRHVRE QSLVTDQLSR
RLIRTYQLYS RTSGKHVQVL ANKRINAMAE DGDPFAKLIV
ETDTFGSRVR VRGAETGLYI CMNKKGKLIA KSNGKGKDCV
FTEIVLENNY TALQNAKYEG WYMAFTRKGR PRKGSKTRQH
QREVHFMKRL PRGHHTTEQS LRFEFLNYPP FTRSLRGSQR
TWAPEPR
|
|
Unit/Weight
|
10 μg
|
|
Unit Price
|
$58.00
|
|
Description
|
Fibroblast Growth Factor 8c (FGF-8c) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. In different species, e.g. human and mouse, FGF-8 has 8 different isoforms, from FGF-8a to FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, thus conduct different signaling cascade pathways. FGF-8 has very widespread expression pattern during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression; on the other hand, FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.
Recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) produced in E. coli is a single non-glycosylated polypeptide chain containing 247 amino acids. A fully biologically active molecule, rmFGF-8c has a molecular mass of 28.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
|
|
Molecular Weight
|
28.2 kDa, observed by reducing SDS-PAGE.
|
|
Purity
|
> 95% by SDS-PAGE and HPLC analyses.
|
|
Storage
|
Lyophilized recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-8c should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
|
|