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Product
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Human mtGPD
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Cat#
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101-5-07A
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Sequence
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His-Tag sequence not included: ADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDD FSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIML PVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQ HNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVR AKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIF FLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIR PLVTDPKSADTQSISRNHVVDISESGLITIAGGKWTTYRSMAEDTINAAVKTHNLKAGPS RTVGLFLQGGKDWSPTLYIRLVQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIV GVRLVSEFPYIEAEVKYGIKEYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELN WDDYKKQEQLETARKFLY
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Unit/Weight
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0.01 mg
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Unit Price
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$228.00
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Description
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Mitochondrial glycerol-3-phosphate dehydrogenase (mtGPD) is a Ca2+-sensitive, FAD-binding protein located on the outer surface of the inner mitochondrial membrane. mtGPD catalyses the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Two isoforms have been described for mtGPD. Isoform 1 comprises 727 aa residues, whereas isoform 2 lacks 126 aa residues of the N-terminus. Deficiency of mGPD appears to contribute to the impairment of glucose-stimulated insulin release in several animal models of non-insulin dependent diabetes mellitus. Recombinant mtGPD comprises a 558 amino acid fragment (43-600) corresponding to the GlpA domain fragment of the mature mtGPD protein and is expressed in E. coli with an amino-terminal hexahistidine tag.
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Molecular Weight
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66.26 kDa
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Purity
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Purity > 95% as determined by reducing and non-reducing SDS-PAGE, Bradford assay, Western blot and ELISA
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Storage
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Store at -20°C. Product is guaranteed one year from the date of shipment.
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