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Product
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HB-EGF, Human
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Cat#
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101-17-07A
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Sequence
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DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG
KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
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Unit/Weight
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10 μg
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Unit Price
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$58.00
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Description
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Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene.HB-EGF-like growth factor is synthesized as a membrane-anchored mitogenic and chemotactic glycoprotein. An epidermal growth factor produced by monocytes and macrophages, due to an affinity for heparin is termed HB-EGF. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. First identified in the conditioned media of human macrophage-like cells, HB-EGF is an 87 amino acid gylcoprotein which displays highly regulated gene expression.Ectodomain shedding results in the soluble mature form of HB-EGF which influences the mitogenicity and chemotactic factors for smooth muscle cells and fibroblasts. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.
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Molecular Weight
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Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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