|
Product
|
IP-10/CXCL10, Mouse
|
|
Cat#
|
101-27-245B
|
|
Sequence
|
IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE
IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP
|
|
Unit/Weight
|
25 μg
|
|
Unit Price
|
$152.00
|
|
Description
|
Interferon Inducible Protein 10 (IP-10)/CXCL10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1β, TNF-α, IL-12 and viruses. Additional cell types that have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human IP-10, Crg-2, has been cloned andshown to share approximately 67% amino acid sequence identity with human IP-10.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. IP-10 has been attributed to several roles, such as chemo attraction for monocytes and T cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.
Recombinant Mouse IP-10/CXCL10 produced in E. coli cells is a single non-glycosylated polypeptide chain containing 77 aminoacids. A fully biologically active molecule, rmIP-10 has a molecular mass of 9.0 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
|
|
Molecular Weight
|
9.0 kDa, observed by reducing SDS-PAGE.
|
|
Purity
|
> 95% as analyzed by SDS-PAGE.
|
|
Storage
|
Lyophilized recombinant Mouse IP-10 /CXCL10 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse IP-10/CXCL10 should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
|
|