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Product
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IL-8 (77aa)/CXCL8, Human
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Cat#
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101-27-218C
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Sequence
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AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA
NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
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Unit/Weight
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1 mg
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Unit Price
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$1343.00
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Description
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Interleukin-8 is one of the first discovered chemokines and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: a 77 a.a. protein produced by endothelial cells, and the more active 72 a.a. protein produced by monocytes. The receptors for IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cell morphology, activate integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells.
Recombinant human Interleukin-8 (IL-8, 77aa)/CXCL8 produced in E. coli is a single non-glycosylated polypeptide chain containing 77 amino acids. A fully biologically active molecule, rhIL-8(77aa)/CXCL8 has a molecular mass of 8.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
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Molecular Weight
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8.9 kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% as analyzed by SDS-PAGE.
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Storage
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Lyophilized recombinant human Interleukin-8 (IL-8)(77aa)/CXCL8 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant human Interleukin-8 (IL-8)(77aa)/CXCL8 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
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