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Product
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SDF-1β/CXCL12, Mouse
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Cat#
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101-27-182A
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Sequence
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KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA
RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM
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Unit/Weight
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10 μg
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Unit Price
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$152.00
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Description
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SDF-1?and SDF-1? members of the chemokine ?subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1?and SDF-1?cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1?and SDF-1?are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1?and absent from SDF-1? SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils. Mice lacking SDF-1 or CXCR4 have been found to have impaired B-lymphopoiesis, myelopoiesis, vascular development, cardiogenesis and abnormal neuronal cell migration and patterning in the central nervous system .
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Molecular Weight
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ximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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