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Product
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TECK/CCL25, Mouse
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Cat#
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101-27-154C
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Sequence
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MQGAFEDCCL GYQHRIKWNV LRHARNYHQQ EVSGSCNLRA
VRFYFRQKVV CGNPEDMNVK RAMRILTARK RLVHWKSASD SQTERKKSNH
MKSKVENPNS TSVRSATLGH PRMVMMPRKT NN
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Unit/Weight
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1 mg
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Unit Price
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$1343.00
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Description
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Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine). It plays a role in the development of T cells and has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Mouse CCL25 shares 49% amino acid sequence identity to with human CCL25.
Recombinant Mouse TECK/CCL25 produced in E. coli is a single non-glycosylated polypeptide chain containing 122 amino acids. A fully biologically active molecule, rmTECK/CCL25 has a molecular mass of 14.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at Pepmic.
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Molecular Weight
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14.3 kDa, observed by reducing SDS-PAGE.
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Purity
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> 98% as analyzed by SDS-PAGE.
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Storage
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Lyophilized recombinant Mouse TECK/CCL25, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse TECK/CCL25 should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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