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Product
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LAG-1/CCL4L1, Human
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Cat#
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101-27-90B
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Sequence
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APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV
VFQTKRGKQV CADPSESWVQ EYVYDLELN
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Unit/Weight
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50 μg
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Unit Price
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$92.00
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Description
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Lymphocyte Activation Gene-1(Lag-1) , also known as C-C motif chemokine 4-like (CCL4L1), is a proinflammatory chemokine and is the isoform of Macrophage Inflammatory Protein-1 beta (MIP-1 beta). LAG-1 was first identified in 1988 and is expressed by monocytes, macrophages, T and B lymphocytes, and dendritic cells upon stimulation. The receptors of LAG-1 are the G-protein coupled receptors CCR1, CCR3, and CCR5 which is its major receptor. LAG-1 is a potent chemoattractant of monocytes, T lymphocytes, dendritic cells, natural killer cells, and activated CD4+ T cells. Unlike CCL4, LAG-1 cannot bind to glycosaminoglycans (GAGs), and thus cannot form an in vivo gradient on the cell surface as does CCL4. Like other members of the MIP-1 subfamily, LAG-1 is associated with several types of disease, including HIV infection and Type I diabetes.
Recombinant human LAG-1/CCL4L1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhLAG-1/CCL4L1 has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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7.8 kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% by SDS-PAGE analysis.
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Storage
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Lyophilized recombinant Human LAG-1/CCL4L1 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human LAG-1/CCL4L1 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
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