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Product
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MIP-1α/CCL3, Human
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Cat#
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101-27-81C
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Sequence
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ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV
IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
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Unit/Weight
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1 mg
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Unit Price
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$1193.00
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Description
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MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1α chemo-attracts macrophages in order to accelerate the tissue repair process.
Recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at Pepmic.
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Molecular Weight
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7.8 kDa, observed by reducing SDS-PAGE.
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Purity
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> 95% as analyzed by SDS-PAGE and HPLC.
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Storage
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Lyophilized recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
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