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Product
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Human LIF
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Cat#
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101-13-138A
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Sequence
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SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
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Unit/Weight
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0.025 mg
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Unit Price
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$228.00
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Description
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Human Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. Human LIF signals through gp130 to activiate STAT3 in human ESCs . In mouse ESCs, LIF promotes self-renewal and pluripotentcy, but in human cells there is no indication that LIF has the same function. Instead, FGF-basic (FGF-2) and Activin A are used to promote self-renewal and pluripotent potential. Human LIF is produced in E. coli. Recombinant human LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 19.9 kDa.
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Molecular Weight
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19.9 kDa
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Purity
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Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
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Storage
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Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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