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Product
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Human MIP-1-beta
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Cat#
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101-13-129A
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Sequence
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APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
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Unit/Weight
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0.01 mg
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Unit Price
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$228.00
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Description
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Macrophage inflammatory protein 1-beta (MIP-1-beta or C-C motif chemokine 4) is a monokine with inflammatory and chemokinetic properties. MIP-1-beta binds to CCR5, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The N-terminal processed form, MIP-1-beta (3-69) retans the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and inhibits CCR5-mediated HIV-1 entry in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant Human MIP-1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids with a MW of 7,620 Da.
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Molecular Weight
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7620 Da
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Purity
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Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
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Storage
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Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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