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Product
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Human TNF-alpha
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Cat#
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101-13-57A
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Sequence
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VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
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Unit/Weight
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0.05 mg
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Unit Price
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$228.00
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Description
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TNF (Tumor necrosis factor) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Genetic variations in TNF are associated with susceptibility to psoriasis and hepatitis B virus infection. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 158 amino acids with a MW of 17,483.77 Da.
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Molecular Weight
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17484 Da
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Purity
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Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
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Storage
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Store at -20°C. Product is guaranteed one year from the date of shipment.
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