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Product
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Human VEGF-121
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Cat#
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101-13-55A
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Sequence
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APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPD EIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRI KPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR
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Unit/Weight
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0.01 mg
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Unit Price
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$228.00
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Description
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Vascular endothelial growth factor-A (VEGF-A) was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways. Alternative splicing of VEGF-A mRNA results in several isoforms of the protein. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. Recombinant Human VEGF-121 produced in E. coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids with a MW of 28,423 Da.
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Molecular Weight
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28423 Da
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Purity
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Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
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Storage
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Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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