|
Product
|
Aak1 Antibody
|
|
Cat#
|
100-21-03A
|
|
Unit/Weight
|
100 μg
|
|
Unit Price
|
$311.00
|
|
Species Reactivity
|
H, M, R
|
|
Tested Application(s)
|
E, WB, ICC
|
|
Brief Description
|
(NT) AP2-associated protein kinase 1
|
|
Specific Description
|
AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
|
|
Storage
|
Aak1 antibody can be stored at 4°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
|
|