Fluorescently Labeled Peptides
Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepmic applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.

Examples of frequently used fluorescent dyes are:

Short name

Full name

Structure

Excitation wavelengths

Emission wavelengths

5-FAM

5-Carboxyfluorescein

492 nm

517 nm

6-FAM

6-Carboxyfluorescein

492 nm

517 nm

Abz

Anthranilyl, 2-Aminobenzoyl

320 nm

420 nm

Cy3

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride

550 nm

570 nm

Cy5

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride

650 nm

670 nm

Dansyl

5-(Dimethylamino) naphthalene-1-sulfonyl

342 nm

562 nm

EDANS

5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid

340 nm

490 nm

FITC

Fluorescein isothiocyanate

490 nm

520 nm

Mca

7-Methoxycoumarinyl-4-acetyl

325 nm

392 nm

Rhodamine B

[9-(2-carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium chloride

553 nm

627 nm

5-TAMRA

Carboxytetramethyl rhodamine


543 nm

572 nm