Polyclonal antibodies(pAb) are produced by inoculation of a suitable mammal, such as a mouse, rabbit, sheep or goat with special designed antigen (purified protein, synthetic peptides, organic compounds or viral particles), which it induces the B-lymphocytes to produce IgG immunoglobulins specific targeting the antigen. This polyclonal IgG is purified from the mammal’s serum. They can be used as both primary and secondary antibodies in Westerns blot, ELISAs, IHCs and other quantitative immune detections. With Pepmic's advanced animal facility, we can provides polyclonal antibody services in mouse, rat, rabbit, chicken, goat/sheep, SPF animals, and other species. Our polyclonal antibody service package includes antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. Customers can simply select their desired packages and send us their specific requirements, and Pepmic will offer antibodies with guaranteed quality of specificity.
Features
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ELISA guarantee >1:40,000
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WB positive guarantee for protein antigen.
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Start as low as $970, deliver as fast as 80 days.
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In-house antigen preparation, including peptide synthesis and gene to protein production service.
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Several animals are available: Rabbits, Goats, Sheep, mice, rats and Guinea Pigs.
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3-5 days express delivery to worldwide customers.
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Polyclonal Antibody Production Packages
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#PEP04200-WB Guaranteed Protein Polyclonal Antibody Package
Antigen: protein( 3-5mg, >85%), customer to supply;
EDT: 3 months;
Price: $1100.00;
Service Contents: protein QC, 2 rabbits immunization, protein A/G purification, ELISA identification.
Guarantees:
ELISA >1:40,000; WB positive.
Deliverables:
1) 15-20mg protein A/G purified antibodies;
2) Pre-immune serum, 0.5 ml/rabbit; Immune serum, 0.5 ml/rabbit;
3) Project report (ELISA data+COA).
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#PEP04210 Peptide Specific Polyclonal Antibody Package
Antigen: peptide(10mg, ≥85%), Pepmic to synthesize;
EDT: as fast as 80 Days;
Price: $970.00;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 2 rabbits immunization, antigen affinity purification, ELISA identification.
Guarantees:
ELISA >1:40,000.
Deliverables:
1) 3-5mg affinity purified antibodies;
2) 1mg peptide, 0.1mg peptide-BSA;
3) Pre-immune serum, 0.5 ml/rabbit; Immune serum, 0.5 ml/rabbit;
4) Project report (ELISA data+COA).
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#PEP04220 Protein Polyclonal Antibody Package
Antigen: 3-5mg protein(＞85%，＞0.5mg/ml), Pepmic to produce;
EDT: 4-5 months;
Price*: $2950.00;
Service Contents: subcloning, protein expression and purification, 2 rabbits immunization, protein A/G purification, ELISA identification
*Customer to provide expression plasmid or Pepmic can start with de novo gene synthesis ($0.35/bp).
*The package price is based on friendly target protein for expression.
Guarantees:
ELISA >1:40,000; WB positive.
Deliverables:
1) 15-25 mg protein A/G purified antibodies;
2) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
3) 0.1mg protein with tag (>85%);
4) Project report (ELISA data+COA).
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#PEP04230 Phospho Specific Polyclonal Antibody Package
Antigen: phospho peptide(15mg, ＞85%), non-phospho peptide(10mg, ＞85%), Pepmic to synthesize;
EDT: 4 months;
Price: $1700.00;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification; ELISA identification.
Guarantees:
ELISA >1:40,000;
DB result - no cross reaction.
Deliverables:
1) 3-5 mg affinity purified phospho antibodies;
2) 3-5 mg affinity purified non-phospho antibodies;
3) 1 mg of phospho & non-phospho peptides; 0.1mg BSA conjugated phospho & non-phospho peptides;
4) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
5) Project report (ELISA + DB data+COA).
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#PEP04240 Formyl Specific Polyclonal Antibody Package
Antigen: formyl peptide(15mg, ＞85%), non-formyl peptide(10mg, ＞85%), Pepmic to synthesize;
EDT: 4 months;
Price: $2650.00;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification, ELISA and DB identification.
Guarantees:
ELISA >1:40,000;
DB result, no cross reaction.
Deliverables:
1) 3-5mg affinity purified formyl antibodies;3-5 mg affinity purified non-formyl antibodies;
2) 1 mg of formyl & non-formyl peptides;
3) 0.1mg BSA conjugated formyl & non-formyl peptides;
4) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
5) Project report (ELISA + DB data+COA).
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#PEP04250 Methyl Specific Polyclonal Antibody Package
Antigen: methyl peptide(15mg, ＞85%), non-methyl peptide(10mg, ＞85%), Pepmic to synthesize;
EDT: 4 months;
Price: $2150.00;
Service Contents: peptide synthesis & KLH conjugation & BSA conjugation, 4 rabbits immunization, antigen affinity purification, ELISA and DB identification.
Guarantees:
ELISA >1:40,000;(methyl specific antibody); DB result - no cross reaction.
Deliverables:
1) 3-5 mg affinity purified methyl antibodies;
2) 3-5 mg affinity purified non-methyl antibodies;
3) 1 mg of methy & non-methyl peptides;
4) 0.1mg BSA conjugated methy & non-methyl peptides;
5) 0.5ml Pre-immune serum & 0.5ml immune serum/rabbit;
6) Project report (ELISA + DB data+COA).
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