Pepmic offers monoclonal antibody (mAb) development in mice and rats, with guaranteed success based on standard endpoints or customer's specific applications. Our high rate of success, short turn-around time and affordable cost are due to our proprietary hybridoma technology, state-of the-art, specific pathogen free (SPF) facility, extensive experience in antibody production for over 10,000 gene targets, and flexibility to suit our customer's needs.
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Monoclonal Antibody Production Package
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#PEP05200-WB Guaranteed Protein Monoclonal Antibody Package
WB guarantee. Uses protein (supplied by customer) as antigen.
Antigen: 10 mg length protein, >90%, Customer to supply.
Cost: $4300. Estimated Timeline: 6 months. Fast protocol is available upon request.
Guarantees:
WB positive; ELISA >1:80,000. You only pay if we deliver with guarantees
Package Deliverables:
1) 2-5 mg antibodies;
2) 2-5 WB positive clones.
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#PEP05210-WB Guaranteed Protein Monoclonal Antibody Package
WB guarantee. Uses protein (produced by Pepmic) as antigen.
Antigen: 10 mg full length protein with tag, >90%, Pepmic to produce.
Cost: $7370. Estimated Timeline: 7-8 months. Fast protocol is available upon request.
Introduction: The Risk-Free package #PEP05210 uses full length protein as antigen with ELISA & WB guarantee. You only pay if we deliver antibodies with guarantees. The package gives the best value for guaranteed WB monoclonal antibodies.
There are 3 stages invovled in this package:
Stage-I: Gene synthesis and subcloning: 2-3 weeks;
Stage-II: Protein expression and purification: 4-5 weeks;
Stage-III: mAb development: 6 months. Fast protocol is available upon request.
Guarantees:
WB positive; ELISA >1:80,000. You only pay if we deliver with guarantees.
Package Deliverables:
1) 2-5 mg antibodies;
2) 2-5 WB positive clones;
3) 0.1 mg protein, >90%.
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#PEP05220-Application Guaranteed Peptide Monoclonal Antibody Package
Application guarantee. uses multiple peptides (synthesized by Pepmic) as antigen.
Introduction: The package targets full length protein, by using up to 21 epitope peptides as antigen for antibody development. Epitope peptide selection is depending on the target protein and the applications.
Production Process, Cost and Timeline
Stage-I: Peptide design, synthesis, antigen preparation and antibody development.
Cost: start from $2500. Estimated Timeline: as fast as 8-9 weeks.
Stage-I delivers ascites samples to you for validation, with Dot Blot guarantee: < 1 ng against the corresponding epitope peptide. You only pay Stage-I cost if we deliver antibody samples with guarantees. You have up to 60 days for antibody validation upon delivery.
Stage-II: Cell line development and ascites production.
Cost: $5470. Estimated Timeline: 4 weeks.
Stage-II is only initiated upon successful antibody validation at Stage-I. If no antibody is validated, customer receives no charge for Stage-II cost.
Application guarantee
Stage-I Deliverables:
1) Ascites samples, 0.2 mg each;
2) Peptides, 0.2-1 mg each.
Stage-II Deliverables:
1) 2 mg purified antibody;
2) 1 cell line.
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#PEP05230-Application Guaranteed Peptide Monoclonal Antibody Package
Application guarantee. uses 1-3 peptides (synthesized by Pepmic) as antigen.
Introduction: The package targets specific sites of the target protein, by using 1-3 epitope peptides as antigen for antibody development.
Production Process, Cost and Timeline
Stage-I: Peptide design, synthesis, antigen preparation and antibody development.
Cost: start from $3100. Estimated Timeline: 6-7 months.
Stage-I delivers ascites samples to you for validation, with Dot Blot guarantee: < 1 ng against the corresponding epitope peptide. You only pay Stage-I cost if we deliver antibody samples with guarantees. You have up to 60 days for antibody validation upon delivery.
Stage-II: Cell line development and ascites production.
Cost: $5500. Estimated Timeline: 4 weeks.
Stage-II is only initiated upon successful antibody validation at Stage-I. If no antibody is validated, customer receives no charge for Stage-II cost.
Application guarantee
Stage-I Deliverables:
1) Ascites samples, 0.2 mg each;
2) Peptides, 0.2-1 mg each.
Stage-II Deliverables:
1) 2 mg purified antibody;
2) 1 cell line.
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#PEP05240-Application Guaranteed Phospho Specific Monoclonal Antibody Package
Application and no cross reaction guarantees. Uses 2 phospho peptides (synthesized by Pepmic) as antigen.
Introduction: The package targets a specific site of modification, by using 2 modified peptides (e.g. phospho specific peptides) as antigen for antibody development.
Production Process, Cost and Timeline
Stage-I: Peptide design, synthesis, antigen preparation and antibody development.
Cost: $4800. Estimated Timeline: 6-7 months.
Stage-I delivers antibody samples to you for validation, with 2 guarantees: 1) Dot Blot < 25 ng against the corresponding epitope peptide; 2) The detection limit of control / immunogen peptide ratio > 100. You only pay Stage-I cost if we deliver with guarantees. You have up to 60 days for antibody validation upon delivery.
Stage-II: Cell line development and ascites production.
Cost: $5450. Estimated Timeline: 4 weeks.
Stage-II is only initiated upon successful antibody validation at Stage-I. If no antibody is validated, customer receives no charge for Stage-II cost.
Application and no cross reaction guarantees
Stage-II Deliverables:
1) Ascites samples, 0.2 mg each;
2) Phospho peptides and control peptides, 0.2-1 mg each.
Stage-II Deliverables:
1) 2 mg purified phospho antibody;
2) 1 cell line.
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