Positional scanning is an important tool for peptide sequence optimization. Two position combinatorial scanning identifies amino acids of interest at two given positions and substitutes them with all other natural amino acids one at a time. Increases in activity will identify the preferred amino acid residues at these positions.
Please input your protein or peptide sequence in one-letter below as well as the position of the two amino acids to be substituted, counting from the N-Terminus. A total of 20 x 20 = 400 sequences will be generated.